DNA constructs of Tandem Repeat, producing Proteins that Attack viruses, fungal and bacterial Plant pathogens through termination of Transcription Factors essential for the above pathogens do their Replication in Plants

摘要

Methods and compositions for the treatment, control, or inhibition of Geminivirus, by reducing or inhibiting the growth of Geminivirus (GV) using a compound having the following structure (1), or a salfarmacu00e9uticamente acceptable that Compound, Charac Bristly because R1, R2, R3 and R4 are selected from the group consisting of a carboxyl Group,A Methyl Group, an Ethyl Group, a group propyl, Isopropyl Butyl Group, a Group, a grupoisobutilo Butyl Group, a secondary, Tertiary Butyl Pentyl Group, a Group, a group Isopentyl Group, a Neopentyl, Fluorine, Chlorine, Bromine, Iodine and Hydrogen.Antivirosicos provides several classes of compounds that can be used parainactivas or disintegrate Geminivirus, such as the African Cassava Mosaic Geminivirus (acmv Geminivirus) and the East African Cassava Mosaic (eamgv), through the attack of the domain D of fingers And Zinc (Zn2 +)] - [CCHC or variations of it,This domain is present in the nucelocu00e1pside or other virosicas PFE. The agents are antivirosicos Design usu00e1rselos in virus infected plants Pathogens. These agents include the u00c1cidopicolu00ednico antivirosicos (PA) and Derivatives of it.Agents can bind Zn2 + coordinated with the virus Protein and expel the PFE of the Zn2 + or Zn2 + and can be linked to form a ternary Complex with PFE PFE Zn2 + - [PA] virus.In addition, also developed a system designed to detect and identify novel acid Derivatives antivirosicos picolu00ednico against GV, based on the ability of these derivatives to react and disrupt the CCHC Motifs (or variations of them) in the PFE of the GV.Novel agents and antivirosicos methods as described in the present Invention, which includes the use of picolu00ednico acid, acid and Derivatives of These aggregates in fusu00e1rico exogenously, also can prevent emplearpara,Control or eliminate any other Plant viruses that contain Essential PFE in the Proteome or use the PFE of the host cell for transcriptional activation. In addition, it discloses a TANDEM Technology secuenciarepetida (TRS), denoted as method of Construct of TRS IN BOX, for the production of plants singu00e9nicas with increased resistance to the virus.This Technology features, in the plant Genome, DNA sequences repetidasen Stable tandem, which encode Enzymes that can produce pa or Derivatives of it. The increased production of PA or Derivatives of it.Increased production of endogenous PA by the picolu00ednico acid carboxylase Promoter, induced protein virosicapor TRS which is present in the Construct of TRS IN BOX, Can inactivate and interrupt the PFE virosicas necessary for virus Replication.Plants in the singu00e9nicas which introduced the Construct of TRS in box for conferirresistencia Viruses are genetically stable and Fertile. Singu00e9nicas TRS plants produce flowers and Seeds that can be planted to produce new plants singu00e9nicas TRS that are resistant to the virus.In addition, the plants singu00e9nicas TRS that obey the present Invention are subject to Evolutionary pressures of the Environment, such as the wild type Counterpart. Moreover, since agents do not contain added exogenously or endogenously, are Edible without having to make any further changes.