Constructions with functional transcriptional Regulatory region of DNA in plant cells and Method for Transforming Aggregates of cells and tissues of plants using micro Needles elongated type

摘要

This is a method to Buddhahood, Transforming plant cells, comprising: (a) select at least cells of Type I, Type II, hipocotil derived Callus derived from Cotyledon, crops or from the Meristem, Pollen, cells or tissues cotyledons of germplasm, and (b) insert DNA In these cells by Transformation mediated by microfibrils.This method is applicable for the transformation of cells and Plant crops, by elongated structures - called "- type Needle Fibers or filaments.Said process includes the agitation of the Aggregates of plant cells and tissues of Plants the plant to be transformed in the presence of DNA and microfibers, through which the DNA uptake and Integration of the same are provided.The process may be applicable to other Aggregates of plant cells and Plant tissues that have proven not to be easily transformable by other techniques.