human fpps x-ray structure and use to select fpps binding compounds

摘要

HUMAN FPPS X-RAY STRUCTURE AND USE TO SELECT FPPS BINDING COMPOUNDS. The present invention relates to crystalline human arnesyl diphosphate synthase (FPPS) for the free FPPS three-dimensional structure as well as the three-dimensional complexes of FPPS with substrates such as IPP (isopentenyl diphosphate) and / or with inhibitors such as like Zometa (r) or Aredia (r). In addition, methods for preparing human FPPS crystals are described. According to the invention crystals can be used to determine the structures of FPPS homologs, mutants, ligand complexes, FPPS crystal forms and similar molecules of unknown structure. The invention further relates to the use of FPPS crystals to select new FPPS ligands, for example by X-ray scanning and to designate and / or identify inhibitors against FPPS. In addition, the invention relates to NMR methods for selecting and / or identifying novel low molecular weight ligands for FPPS which represent novel therapeutic agents.