Establishment of cell line of human stem embrionu00c1rias using mammalian cell

摘要

Establishment of cell line of human stem embrionu00c1rias using mammalian cell, where they are revealed to purified preparations of human embryonic stem cells with certain specific characteristics of the population.This preparation is characterized by a positive expression of the following markers of pluripotent cell surface: SSEA - 1 (-); SSEA - 4 (+); tra - 1 - 60 (+); tra 1 81 (+); alkaline phosphatase (+), as well as a set of cellular markers es including OCT 4, Nanog, rex1, Sox2, THY1, Fgf4, ab Group CG2, dppa5, utf1, cripto1, hTERT, connexin 43, connexin 45.The cells are negative for the preparation of lineage specific markers such as keratin 8, Sox - 1, NFH (ectoderm), MyoD, Brachyury, cardiac actin (mesoderm), HNF - 3 beta, albumin and PDX1 (entoderm).The cells of the preparation are embryonic stem cells have normal karyotypes of human, exhibit high activity of telomerase and continue to proliferate in an undifferentiated state after continuous culture of more than 40 passages.The lineage of embryonic stem cells relicellu00ae hes 1 also has the skill, throughout the culture, to differentiate the types of tissues and cells derived from all three layers of embryonic origin (endodermis, mesoderm and ectoderm). Also disclosed are methods for isolating the line of human embryonic stem cells.