Method for diagnu00e0stico and discriminau00c7u00e7o strains of Eimeria Eimeria tenella and acervulina, Eimeria Maxim based on comparau00c7u00e7o of haplu00e0tipos of loci markers amplified with primers specific to the microssatu00c9lites said.

摘要

Method for diagnosis and discrimination of strains of Elmeria acervulina, Elmeria tenelia and Elmeria maximum based on comparison of the haplotypes of loci microsatellites amplified with specific primers for markers of said method.The present invention relates to a method to diagnose and distinguish different strains of Elmeria ace, vullna, Elmeria tenelia and Elmeria maxima, species of protozoa that cause parasitic infections in the intestine of domestic fowl.The method is based on molecular markers of the type microssatu00e9lite and consists of: (a) the purification of DNA from oocysts of parasites obtained from samples of feces; (b) DNA amplification using primers (Primers) specific and unique through reaction technique in C Adela of polymerase (PCR); (c) the separation of the products of amplification by electrophoresis; (d) the identification of productionBands amplified by molecular size; and (E) a comparison of haplotypes (combinations of alleles present in the different Ioci) of the samples tested.