The process of obtenu00c7u00e7o to transform yeast strains containing mu00e9ltiplas cu00e0pias stable gene inserted in the genome of informau00c7u00e7o desired

摘要

The process of obtenu00c7u00e7o to transform yeast strains containing mu00e9ltiplas cu00e0pias stable of informau00c7u00e7o desired gene inserted in the genome. The present application consists in the development of a new process that allows for the genetic transformation of strains of the yeast Saccharomyces spp from the culture collection of lab and yeasts employed in industrial processes.To do so, a genetic transformation vector was constructed that consists of a DNA fragment of double strand containing a yeast gene expression cassette flanked by fragments corresponding to the regions of the transposons franqueadoras Ty1 and Ty2.These DNA fragments are long terminal regions ("long terminal page" - LTR elements) that are called Delta ( sym ) and are present in more than 200 copies in the genome of yeast cells.To construct the new system of transformation, it was important to use, as an example, a gene reporter has confirmed the success of transformation, through the verification of the insertion of the genetic information required in the genome of yeast. To do so, was the chosen cDNA of glicoamilase of aspergilius awamori, building up the vector p Glico sym sym.