method of quantifying 24S - u043cu0435u0442u0438u043bu0431u0440u0430u0441u0441u0438u043du043eu0441u0442u0435u0440u043eu0438u0434u043eu0432 (u0431u0440u0430u0441u0441u0438u043du043eu043bu0438u0434u0430 and u043au0430u0441u0442u0430u0441u0442u0435u0440u043eu043du0430) and composition for it

摘要

the invention belongs to the field of u0438u043cu043cu0443u043du043eu0445u0438u043cu0438u0447u0435u0441u043au043eu0433u043e analysis, namely quantifiable u0444u0438u0442u043eu0433u043eu0440u043cu043eu043du043eu0432, plant growth regulators, 24S - u043cu0435u0442u0438u043bu0431u0440u0430u0441u0441u0438u043du043eu0441u0442u0435u0440u043e u0438u0434u043eu0432 - u043au0430u0441u0442u0430u0441u0442u0435u0440u043eu043du0430 and u0431u0440u0430u0441u0441u0438u043du043eu043bu0438u0434u0430,and is designed to determine the u0431u0440u0430u0441u0441u0438u043du043eu0441u0442u0435u0440u043eu0438u0434u043eu0432 in aqueous solutions, and also can be used for quantitative assessment of biological objective tah.determination of residue in the soil, wastewater and the like.the present invention is a method of quantifying 24S - u043cu0435u0442u0438u043bu0431u0440u0430u0441u0441u0438u043du043eu0441u0442u0435u0440u043eu0438u0434u043eu0432 (u043au0430u0441u0442u0430u0441u0442u0435u0440u043eu043du0430 and u0431u0440u0430u0441u0441u0438u043du043eu043bu0438u0434u0430) and composition for implemented whoa, which differ in specificity.high sensitivity, environmental security, simplicity, reliability and process efficiency.the goal is achieved, firstly, the proposed methodensure the mixing of the analysed samples u0431u0440u0430u0441u0441u0438u043du043eu0441u0442u0435u0440u043eu0438u0434u0430 (or calibration samples) with u043au043eu043du044au044eu0433u0430u0442u043eu043c u043au0430u0441u0442u0430u0441u0442u0435u0440u043eu043d - horseradish peroxidase in the polymer (e.g.u043fu043eu043bu0438u0441u0442u0438u0440u043eu043bu044cu043du044bu0445) u043bu0443u043du043au0430u0445 with u0438u043cu043cu043eu0431u0438u043bu0438u0437u043eu0432u0430u043du043du044bu043cu0438 on the inner surface of u043fu043eu043bu0438u043au043bu043eu043du0430u043bu044cu043du044bu043cu0438 antibodies to u043au0430u0441u0442u0430u0441u0442u0435u0440u043eu043du0443, subsequent incubation.separation of free and bound forms of u0431u0440u0430u0441u0441u0438u043du043eu0441u0442u0435u0440u043eu0438u0434u043eu0432 antibodies, an addendum to the bound form u0445u0440u043eu043cu043eu0433u0435u043d - u0441u0443u0431u0441u0442u0440u0430u0442u043du043eu0439 mixtures to determine the activity u043fu0435u0440u043eu043au0441u0438 u0434u0430u0437u044b,on that count the number of defined 24S - u043cu0435u0442u0438u043bu0431u0440u0430u0441u0441u0438u043du043eu0441u0442u0435u0440u043eu0438u0434u0430; secondly, composition for its implementation, including antibodies to u043au0430u0441u0442u0430u0441u0442u0435u0440u043eu043du0443,u0438u043cu043cu043eu0431u0438u043bu0438u0437u043eu0432u0430u043du043du044bu0435 on polymeric u043bu0443u043du043au0430u0445; u043au043eu043du044au044eu0433u0430u0442 u043au0430u0441u0442u0430u0441u0442u0435u0440u043eu043du0430 with u043fu0435u0440u043eu043au0441u0438u0434u0430u0437u043eu0439 hell; buffer solution for breeding u043au043eu043du044au044eu0433u0430u0442u0430; u0445u0440u043eu043cu043eu0433u0435u043d - u0441u0443u0431u0441u0442u0440u0430u0442u043du044bu0439 buffer (u0440u0430u0441u0442u0432 or 3,3 ', 55 '- u0442u0435u0442u0440u0430u043cu0435u0442u0438u043bu0431u0435u043du0437u0438u0434u0438u043du0430 in u0441u0443u0431u0441u0442u0440u0430u0442u043du043eu043c buffer with hydrogen peroxide