SUPER PARAMAGNETIC NANOPARTICLES BASED ON IRON OXIDES WITH A MODIFIED SURFACE, METHOD FOR THEIR RECEPTION AND APPLICATION

摘要

For marking cells, it is proposed to use samples of superparamagnetic nanoparticles with a narrow size range, prepared by prepositioning colloidal Fe (OH) by treating an aqueous solution of Fe (III) salt with a small content of NHOH, to which a solution of Fe (II) salt is added, and an excess of NHOH is added to the mixture . After aging, magnetic separation and washing, sodium citrate is added to the mixture and then an aqueous solution of sodium hypochlorite until the formation of colloidal maghemite, to which is added an aqueous solution of the modifying agent. The modifying agent includes mono-, di- or polysaccharides from the group consisting of D-arabinose, D-glucose, D-galactose, D-mannose, lactose, maltose, dextran and dextrins or amino acids or polyamino acid from the group formed by alanine, glycine, glutamine, asparagine, histidine, arginine, L-lysine, aspartic or glutamic acid. The polymers of (meth) acrylic acid derivatives are selected from the group comprising poly (N, N-dimethyl acrylamide), poly (N, N-dimethyl methacrylamide), poly (N, N-diethyl acrylamide), poly (N, N-diethyl methacrylamide), poly ( N-isopropylacrylamide), poly (N-isopropyl methacrylamide).