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method deutereeritud bl21 su0094u0094tmes escherichia coli fed batch cultivation (de3) and recombinant protein ekspressiooniks
method deutereeritud bl21 su0094u0094tmes escherichia coli fed batch cultivation (de3) and recombinant protein ekspressiooniks
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机译:补料分批培养(de3)和重组蛋白ekspressiooniks的方法deutereeritud bl21 s u0094 u0094tmes
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摘要
A method for cultivation of E.coli BL21 and its derivatives in deuterated media in fed-batch culture and expression of recombinant DNA products is described. The method is based on the usage of inexpensive deuterated carbon sources succinate and acetate together. The simultaneous supply of these substrates at molar ratio of 4-8 (mole/mole) acetate/succinate to the culture enables good growth to high cell densities and expression in fed-batch culture. A specific fed-batch control algorithm is applied to automatically adjust the feeding rate to the rate of the utilization of carbon sources based on dissolved oxygen control.
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