A METHOD AND APPARATUS FOR DETECTING SUBSTANCE TO BE DETECTED CONTAINING AT LEAST ONE COMPONENT THAT IS DISPERSIBLE IN AIR IN THE FORM OF SOLID PARTICLES

• 1. 一种空气中固体颗粒浓度检测装置 [P] . 中国专利： CN213022757U . 2021-04-20
• 2. 用于制造用于检测测量介质中的至少一种气态分析物的气体传感器设备的方法以及用于检测测量介质中的至少一种气态分析物的方法和气体传感器设备 [P] . 中国专利： CN107250783A . 2017-10-13
• 3. A METHOD AND APPARATUS FOR DETECTING SUBSTANCE TO BE DETECTED CONTAINING AT LEAST ONE COMPONENT THAT IS DISPERSIBLE IN AIR IN THE FORM OF SOLID PARTICLES [P] . IL192155A . 2009-05-04

  机译： 用于检测要检测的至少包含一种在固体中以固体颗粒形式存在于空气中的成分的方法和装置

• 4. Method and apparatus for detecting substance to be detected containing at least one component that is dispersible in air in the form of solid particles [P] . 美国专利： US2004202574A1 . 2004-10-14

  机译： 用于检测包含至少一种以固体颗粒形式可分散在空气中的成分的被检测物质的方法和设备

• 5. Method for detecting substance to be detected containing at least one component that is dispersible in air in the form of solid particles [P] . 美国专利： US7846742B2 . 2010-12-07

  机译： 包含至少一种以固体颗粒形式可分散在空气中的成分的待检测物质的检测方法

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• 2. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
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