POT STAINING OF SPUTUM FOR DETECTION OF ACID FAST BACILLI

摘要

The present invention relates to a process to sterilize and stain the sample in its container before making a smear. Sputum samples are treated in its container with one ml of phenol ammonium sulphate basic fucshin solution. A flocculate-smear is made after 1 hour from the stained and cooled sample. The smear was decolourised by sulphuric acid and counter stained by melhylene blue before examination.