A PROCESS OF FORMULATION OF NEEM (AZADIRACHTA INDICA) ROOT BASED NATURAL FUNGICIDAL PRODUCT

摘要

Collecting neem root from Banaras Hindu University Campus and drying it at room temperature(25±2°C). Crushing 4 Kg air dried roots in a pastel and mortar to make powder. Extracting twice with 8 litres of methanol in a soxhlet at (50 ± 5°C). Evaporating the solvent by steam distillation to get dry extract. Dissolving the crude extract in methanol in the proportion of 1:5(W/V). Treating the solvent with charcoal and filtering the supernatant through whatman nol. filter paper. Evaporating the excess solvent and finally drying it under vaccum. Passing the so obtained extract of neem root through si-gel column for clean up after washing the column through methanol to get refined neem root extract. Evaporating the solvent under vacuum to get semi solid yellowish brown neem root extract. Formulating this extract in water containing 0.1% tween 20 in the concentration of 250,500,1000,2000,2500 and 3000 ppm(Parts per million) for the purpose of testing its fungicidal effect against phytopathogenic and saprophytic fungi and also powdery mildew disease of pea( Pisum Sativum ).