A RECOMBINANT SUBGROUP B ADENOVIRUS PARTICLE COMPRISING AN ADENOVIRUS GENOME

摘要

ABSTRACT 2401/CHENP/2004 "A recombinant subgroup B adenovirus particle comprising an adenovirus genome" The present invention relates to a recombinant subgroup B adenovirus particle comprising an adenovirus genome wherein the genome comprises a deletion in the El-region, which adenovirus genome does not encode a functional E1B 55K gene product, said adenovirus genome comprising a pIX coding sequence under control of an expression sequence, said expression sequence comprising part of the 3' end of the E1B 55K coding sequence containing from 150 to 600 nucleotides of adenovirus sequences directly upstream of the pIX open reading frame of a wild-type subgroup B adenovirus, and said expression sequence being capable of increasing expression of the pIX coding sequence in a packaging cell, relative to the expression of said pIX coding sequence when preceded by sequences between the E1B 55K stop codon and the start codon of pIX and without nucleotides from the 3' end of the E1B 55K coding sequence.