HUMAN ORAL CANCER CELL LINE ESTABLISHED AND PROPAGATED IN VITRO FROM ORAL

摘要

This invention relates to a method of producing human oral cancer cell line which comprises, subjecting oral squamous cell carcinoma from the floor of mouth to the step of biopsy in Hanks Balanced Salt Solution (HBSS) as a buffer supplemented with antibiotics (Penicillin and streptomycin) and amphotaricin B, cutting the treated tissue of step (a) into smaller pieces, washing the cut tissues with solution of antibiotics, the washed tissues being introduced into tissue culture flasks having a medium comprising DMEM and media 199 in a ratio of 2:1 supplemented with fetal bovine serurn (FBS) and growth supplements such as herein described to allow the growth of cells comprising fibroblast cells which grow earlier than the epithelial cells, removing the fibroblasts cells from the culture to obtain a cell line comprising essentially of epithelial cells.