method and kit for tierartspezifischen dna identification in a sample

摘要

The invention relates to a method for detecting the presence or absence of animal species-specific DNA in a sample, which comprises the following steps: (a) providing suitable DNA from the sample for the purpose of polymerase chain reaction (PCR), (b) carrying out a PCR reaction with the DNA of step (a) using a primer mixture that comprises a labeled marker for the purpose of producing a PCR product, the primers hybridizing to preserved regions of a gene ubiquitary in the animals to be identified and the PCR product having a region with an animal species-specific sequence that is variable in all animal species or animal varieties of interest, (c) immobilizing the PCR product of step (b) that contains labeled DNA sequences, (d) producing immobilized single-stranded DNA of the immobilized PCR product from step (c), (e) treating the immobilized single-stranded DNA from step (d) under hybridization conditions with at least one animal species-specific probe, (f) detecting if hybridization has taken place in step (e) in order to detect the presence or absence of the animal species-specific DNA.