Cloning and characterization of napsin, an aspartic protease.

摘要

The present invention REFERS TO cloned FROM A BANK OF HUMAN LIVER A previously unknown aspartic protease, CAPABLE OF CONDUCTING A CRACK Hydrolysis PROTEIN AND CALLED AHEAD "napsin". Have been cloned, sequenced and expressed DOS CLONES ENCODING DNA SUPPLEMENTARY ISOENZYMES PROTEASE CALLS "napsin A" Y "napsin B". ALSO it has been obtained and partially sequenced the gene. ALSO it HAS DEVELOPED a purification process FAST THANKS TO ENZYME IMMOBILIZED pepstatin and has been isolated an enzyme TISSUE FROM HUMAN RENAL. They HAVE OCCURRED Polyclonal antibodies against ENZYMES, ALLOWING THESE antibodies also isolate and detect the enzyme. SIMILARITIES WITH aspartic other proteases, especially cathepsin D, demonstrate the utility of the enzyme DIAGNOSTICS PROCEDURES, AND PROTEASE. You can determine the amount and / or type of napsin TISSUE EXPRESSED IN A PARTICULAR using oligonucleotide probes O AGAINST napsin labeled antibodies.