Use of a restriction enzyme to isolate iii type sequence tags comprising more than 25 nucleotides.

摘要

Using a restriction enzyme type III to isolate, with respect to the cDNA of an expressed gene a tag comprising more than 25 nucleotides and capable of identifying the expressed gene, wherein the 3 '' label it is defined by a cleavage site of restriction enzyme type III, and the 5 '' of the tag is defined by the cleavage site of another restriction enzyme that is closest to the 3 '' of the cDNA expressed gene.