A method of fixing-dehydration-removing fat tissue-pervading continuous production of high quality.

摘要

A method for continuous tissue processing comprising (a) providing a sample of tissue up to 3 mm thick; (B) fixing, dehydrating and removing fat from said tissue specimen with microwave energy and non-aqueous solutions, at least one nonaqueous solution a first solution comprising an alcohol and a ketone in a volume ratio of alcohol with respect to ketone between 1 and 3; (C) impregnating said sample of tissue fixed, dehydrated and defatted in subatmospheric pressure by a wax solution comprised of paraffin; (D) repeating steps (a) to (c) with at least another tissue specimen; whereby they treated continuously tissue samples initiating step (a) for a sample of treated fabric subsequently before step (c) is completed for a sample of treated fabric above so that a tissue sample is from fixation to impregnation in less than two hours.