Method for the regeneration of neurites from adult and agricultural neurons of the spiral gangly. (Machine-translation by Google Translate, not legally binding)

摘要

Method for the regeneration of neurites from adult and aged neurons of the spiral ganglion. The present invention relates to a method for the regeneration of neurites from adult and aged neurons of the spiral ganglion that includes both a mechanical and an enzymatic dissociation with trypsin at 37º c, the adhesion of the explants to positively charged surfaces and a conditioned culture medium that incorporates horse serum, l-glutamine, supplements b27 and n2 simultaneously and growth factor gap-43. The invention can be applied in regenerative medicine to solve problems related to hearing loss and different sensorineural deafness including presbycusis. (Machine-translation by Google Translate, not legally binding)