METHOD FOR ENHANCEMENT OF NATURALLY OCCURRING CYTOPLASMIC MALE STERILITY AND FOR RESTORATION OF MALE FERTILITY AND USES THEREOF IN HYBRID CROP PRODUCTION

摘要

The present invention relates to methods for enhancement of naturallyoccurring cytoplasmic male sterility and for restoration ofmale fertility and uses thereof in hybrid crop production. There is alsodisclosed a method for restoration of male fertility to cytoplasmicmale sterile plants; which comprises the steps of: a) introducing into thenucleus of a plant cell a gene construct essentially consisting of asequence encoding a mitochondrial transit peptide fused upstream of and inframe with an edited form of a normal mitochondrial gene thatis co-transcribed with an unusual CMS-associated mitochondrial gene; b)selecting for plan cells that have acquired the gene construct instep a); and c) inducing regeneration of selected plant cells to produce amature plant.