DIAGNOSIS OF METAL ALLERGY THROUGH CYTOKINE RELEASE BY T-CELLS IN VITRO

摘要

An in vitro method for diagnosing a contact allergy in a subject comprises:(i) providing (a) a contact allergen; (b) T-cells from said subject; and (c) asurface to which is immobilised a first specific binding agent capable ofspecifically binding to a cytokine released from a T-cell in response to saidallergen; (ii) contacting said sample with said allergen under conditionswhich are suitable for (a) release of said cytokine by T cells that have beenpresensitised in vivo to said allergen; and (b) binding of said cytokine tosaid first specific binding agent; and (iii) detecting binding of saidsubstance to said first specific binding agent. A kit which enables the methodto be carried out is also described.