A NON-DESENSITIZING MUTANT OF THE TRANSIENT RECEPTOR POTENTIAL TRPM5 ION CHANNEL

摘要

There exists a need in the art for high throughput screening assays that canidentify compounds that specificallymodulate the activity of fast-acting ion channels, such as TRPM5. Currentmethods, especially electrophysiological, suffer froma lack of sensitivity, rapid signal loss, low throughput, and are laborintensive. The claimed methods and compositions provideelectrophysiology methods that allow prolonged sample testing and fluorescentassays with an optical readout that gives rapid readoutof the results, has a high signal to noise background ratio, are easy to use,can be modified for automation and miniaturization, andprovide verification that a compound specifically modulates TRPM5.