A METHOD FOR PREPARING THE PRIMERS FOR IN VITRO IDENTIFYING MUTATIONS WITHIN LARGED VESTIBULAR AQUEDUCT SYNDROMIC HEARING LOSS PDS GENE AND THE USE THEREOF

摘要

A method for preparing the primers which can be used to in vitro identify mutations within larged vestibular aqueduct syndromic hearing loss PDS gene. The method comprises designing the primer pairs which can insert any substitution base at the region consisting of 1 to 13 bases which comprising the site IVS7-2A?G and part of the upstream and downstream regions thereof, according to the mutation site IVS7-2A?G within PDS gene, and then obtaining new restriction endonuclease site from the amplified product which can be used to identify IVS7-2 wild type A site and the mutant G. The primers produced by the above method, kit, agent or the like, which comprising the primers, and the method for identifying the mutation which comprising the larged vestibular aqueduct syndrome IVS7-2A?G are also provided.