PROCESS FOR THE ISOLATION OF PLACENTA-DERIVED TROPHOBLAST STEM CELLS

摘要

The present invention provides a method for isolating trophoblast stem cells, the method including: (a) harvesting placental villi from a detached normal placenta; (b) adding an enzyme solution containing trypsin, DMase I, and dispase to the placental villi of step (a) to perform an enzymatic reaction and adding a fetal bovine serum thereto to terminate the enzymatic reaction; (c) centrifuging the reaction solution of step (b) and separating cytotrophoblasts from the recovered cells by a density-gradient separation method; and (d) culturing the cytotrophoblasts of step (c) in a medium containing a fetal bovine serum and an antibiotic.