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METHOD FOR ISOLATING RENAL STEM/PROGENITOR CELL, RENAL STEM/PROGENITOR CELL AND THERAPEUTIC AGENT FOR RENAL DISEASE

机译:分离肾干/祖细胞的方法,肾干/祖细胞和用于视网膜疾病的治疗剂

摘要

It is intended to provide a method for noninvasively isolating a human renal stem/progenitor cell, an isolated renal stem/ progenitor cell, a therapeutic agent for renal disease, a mouse mesenchymal cell which can be used for isolating a human renal stem/progenitor cell and a culture supernatant of the same. A renal stem/progenitor cell is isolated by primarily culturing a cell contained in the urine of a patient with renal disease in a medium containing a mouse mesenchymal cell identified by the deposition number of FERM ABP-10865 or a culture supernatant of the same, staining the obtained primarily cultured cell with Hoechst 33342 and separating a weak-positive or negative fraction.
机译:本发明旨在提供一种用于无创地分离人肾干/祖细胞,分离的肾干/祖细胞,用于肾脏疾病的治疗剂,小鼠间充质细胞的方法,所述方法可用于分离人肾干/祖细胞。以及相同的培养上清液。肾干/祖细胞的分离是通过首先在含有小鼠间充质细胞的培养基中培养肾病患者尿液中所含的细胞,所述小鼠间充质细胞通过FERM ABP-10865的沉积号或该培养物的上清液进行染色,用Hoechst 33342获得的原代培养细胞,并分离出弱阳性或阴性部分。

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