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GENETICALLY ENCODED CALCIUM SENSORS COMPRISING THE C-TERMINAL LOBE OF TROPONIN C AND A FLUORESCENCE TAG
GENETICALLY ENCODED CALCIUM SENSORS COMPRISING THE C-TERMINAL LOBE OF TROPONIN C AND A FLUORESCENCE TAG
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机译:遗传编码的钙传感器,包含肌钙蛋白C的C末端波瓣和荧光标记
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摘要
The present invention relates to genetically encoded calcium sensors comprising fluorescent proteins and troponin C as calcium-binding moiety. More specifically, the invention provides a polypeptide comprising a donor moiety for fluorescence resonance energy transfer (FRET), at least two calcium binding moieties derived from the C-terminal domain of troponin C, and an acceptor moiety for FRET. Also, the invention provides nucleic acid molecules, expression vectors, host cells, and transgenic animals. In addition, methods for detecting a change in Ca2+ concentration or Mg2+ concentration, as well as uses of the polypeptides for preparing diagnostic compositions for the detection of changes in the Ca2+-concentration or Mg2+-concentration are provided.
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