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BI-DIRECTIONAL SELECTION MARKERS WITH IMPROVED ACTIVITY
BI-DIRECTIONAL SELECTION MARKERS WITH IMPROVED ACTIVITY
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机译:具有改善的活动的双向选择标记
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摘要
The present invention discloses a polypeptide selected from the group consisting of: a polypeptide having an amino acid sequence according to SEQ ID NO 3, a polypeptide having an amino acid sequence according to SEQ ID NO 6, a polypeptide having an amino acid that is substantially homologous to the sequence of SEQ ID NO 3 and a polypeptide having an amino acid that is substantially homologous to the sequence of SEQ ID NO 6, the polypeptide displaying acetamidase activity and providing a reverse selection on fluoroacetamide with an efficiency of at least 95%, preferably at least 96%, more preferably at least 97%, more preferably at least 98%, more preferably at least 99%, most preferably 100%. The gene encoding the polypeptide of the invention is used as an efficient bi-directional selection marker in the construction of selection marker free strains, in particular for processes for the production of a compound of interest.
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机译:本发明公开了选自以下的多肽:具有根据SEQ ID NO 3的氨基酸序列的多肽,具有根据SEQ ID NO 6的氨基酸序列的多肽,具有基本上为氨基酸的氨基酸的多肽。与SEQ ID NO 3的序列同源并且具有与SEQ ID NO 6的序列实质上同源的氨基酸的多肽,该多肽表现出乙酰胺酶活性并以至少95%的效率提供对氟乙酰胺的反向选择,优选至少96%,更优选至少97%,更优选至少98%,更优选至少99%,最优选100%。编码本发明多肽的基因在构建无选择标记的菌株中,特别是用于生产目的化合物的方法中,用作有效的双向选择标记。
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