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TRANSFECTION AND TRANSFER OF NON-HUMAN MALE GERM CELLS FOR GENERATION OF TRANSGENIC NON-HUMAN MAMMALS
TRANSFECTION AND TRANSFER OF NON-HUMAN MALE GERM CELLS FOR GENERATION OF TRANSGENIC NON-HUMAN MAMMALS
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机译:非人类人类生殖细胞的转化和转移,以产生转基因非人类哺乳动物
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摘要
A composition for in vivo transfection of vertebrate male germ cells comprises a nucleic acid or transgene, and a gene delivery system, and optionally a protective internalizing agent, such as an endosomal lytic agent, a virus or a viral component, which is internalized by cells along with the transgene and which enhances gene transfer through the cytoplasm to the nucleus of the male germ cell. A pharmaceutical preparation and a transfer kit utilize the composition. A method for introducing a polynucleotide into vertebrate male germ cells comprises the administration of the composition to a vertebrate. A method for isolating or selecting transfected cells utilizes a reporter gene, and a method for administering transfected male germ cells utilizes male germ cells which have been transfected in vitro.
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