HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC (HPLC) PURIFICATION OF PROTEINS USING SEMI-COMPRESSIBLE RESINS

摘要

A process for the purification of a protein and, in particular a monoclonal antibody, is described which involves the use of a semi-compressible resin in a high performance liquid chromatography column operated at high flow rates and operating pressures. The process can be used for all stages of protein purification including protein A affinity chromatography, anion exchange chromatography and cation exchange chromatography.