The invention provides a method for producing lentiviral vectors for site-specific delivery of nucleic acid molecules based on the bacteriophage intigrase-mediated recombination and Vpr delivery technology, wherein the lentiviral vectors deliver and Integrate the carried nucleic acid molecules into a target cellular genome in a site-specific manner. The invention further provides a lentiviral vector system comprising a transducing vector construct that contains a target sequence of an enzyme-mediated sequence-specific homologous recombination, a construct expressing a recomblnase gene (used to a lentiviral particle-associated accessory gene, a construct expressing lentlvlral gag-pol polyprαteln and a construct expressing viral envelope protein.
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