Method of Modifying the Genome of Gram-Positive Bacteria by means of A Novel Conditionally Negative Dominant Marker Gene

摘要

The present invention is Gram-it relates to a novel method, such a bacteria and the new vector to transform the genome of a positive bacteria. In particular, the invention relates to a method of modifying the stiffness four bacterial or non-bacterial breather using a novel marker gene having the dominant negative conditional functional in bacteria. ; Gram-positive bacteria, conditional voice dominant marker gene, sacB gene, Yerevan can Kraze.