TWO PHOTON PROBE FOR REAL TIME MONITORING OF CELLS, METHOD FOR PREPARING THE SAME AND METHOD FOR REAL TIME MONITORING OF CELLS

摘要

Two-photon dye for real-time monitoring of cells, in which the charge transport within molecule is facilitated because of having high deltaTPA by holding strong donor-acceptor pairs within the annular planar structure, the optical stability is excellent because of having the conjugated bond within the loop, the water solubility is excellent because of having the integrated ester as receptor, and the cell permeability is superior because of having a small molecular weight, is provided. And a preparing method of the two-photon dye and a real-time cell monitoring method using the same are also provided. Two-photon dye for real time monitoring of cells is represented by a formula 1. In the formula 1, X is -OCH3, and -OH or - N(CH3) 2, and Y is -COOCH3, and -CN or -COOC4H4NO2. The maximum wavelength of the two-photon excitation spectrum of the compound in the formula 1 ranges from 780nm to 940nm.