Human Unshared Protein-2 Monobasic Polymorphism Marker for Predicting Risk of Atherosclerosis and Vascular Disease

摘要

UCP-2(uncoupling protein-2) SNP(single nucleotide polymorphism) makers for the prediction of susceptibility to coronary heart disease and atherosclerosis are provided to analyze correlation between the UCP-2 SNP and obesity, especially indexes of blood cholesterol and atherosclerosis. The UCP-2 SNP makers for the prediction of susceptibility to coronary heart disease and atherosclerosis are -1957GA SNP marker in which a -1957th base from the transcription-starting point of the UCP-2 gene(87th base of SEQ ID NO:1) is G, -866GA SNP marker in which a -866th base from the transcription-starting point of the UCP-2 gene(63th base of SEQ ID NO:2) is G, +4787CT SNP marker in which a 4784th base from the transcription-starting point of the UCP-2 gene(53th base of SEQ ID NO:3) is C, and +791_45di SNP marker in which 45 bp bases from 7941th base from the transcription-starting point of the UCP-2 gene(45 bp bases from 253th base of SEQ ID NO:4) are added or deleted. A UCP2 haplotype marker for the prediction of susceptibility to coronary heart disease, in which -866th and 4787th bases from the transcription-starting point of the UCP-2 gene are G and C, respectively, is also provided. A method for the prediction of susceptibility to coronary heart disease and atherosclerosis comprises the steps of: (1) isolating the genomic DNA from a blood sample; (2) detecting the UCP-2 SNP site from the isolated genomic DNA; and (3) confirming the detected UCP-2 SNP site.