THE ENDO-GLUCANASES FROM THE BROWN-ROT BASIDIOMYCETE FOMITOPSIS PALUSTRIS HAVING MICROCRYSTALLINE CELLULOSE DEGRADING ACTIVITY

摘要

It is arranged to develop wood-biomass lyase wood-biomass resolving system and production technology from the endoglucanase of fomitopsis palustris. Contain amino acid sequence ALFGLMNEPH and AAGAWNYLLL from an endoglucanase EG47 derivatives of fomitopsis palustris, molecular weight is 47kDa. Endoglucanase has microcrystalline cellulose dissolution activity. Wood-biomass lyase includes from dextranase EG 47 or from dextranase EG 35. Research for endoglucanase includes: the step from fomitopsis palustris cleaning glycosylhydrolases; One step of enzyme analysis and protein concentrate; Pass through a step of the enzyme of analysis of amino acid sequence identification purifying; Analyze a step of the characteristic of pure endoglucanase; The step analyzed with chromatography.