METHOD FOR LARGE SCALE PREPARATION OF THE PHOSPHORYLATED AND ACTIVE FORM OF EXTRACELLULAR-REGULATED KINASE IN E. COLI EXPRESSION SYSTEM

摘要

The present invention is extracellular adjusting kinases (the phosphorylated activated form of extracellular-adjusting kinases; Hereafter, ERK) he is related to manufacturing method, and particularly, map/ERK kinases is represented as a fusion protein, has in Escherichia coli E.(the GST in MAPK/ERK kinases 1 of table 1 of coli systems connection; MEK1) and the co- by expressing ERK is that the phosphorylated ERK activity class being overexpressed is related to. The ERK that the phosphorylated activated form of overexpression is of the invention is cancer; stroke; when seeking new drug development, it is conducive to be used as the standard substance for determining inhibitor complex investigation and research to cell function curative drug as the drug development target protein high-efficient for neuropathy.