首页> 外国专利> AMINO ACID DEAMINASE GENE OF PROTEUS MIRABILIS, PROTEIN, RECOMBINANT VECTOR AND TRANSFORMANT, AND METHOD FOR MANUFACTURING FUNCTIONAL ORGANIC ACIDS USING THE SAME

AMINO ACID DEAMINASE GENE OF PROTEUS MIRABILIS, PROTEIN, RECOMBINANT VECTOR AND TRANSFORMANT, AND METHOD FOR MANUFACTURING FUNCTIONAL ORGANIC ACIDS USING THE SAME

机译:变形杆菌,蛋白质,重组载体和转化子的氨基酸脱氨酶基因,以及使用其制造功能性有机酸的方法

摘要

The amino acid deaminase gene that one proteus mirabilis derives from is provided to the various functional organic acid of large-scale production by bioconversion with amino acid substrate. One gene has the activity for the amino acid deamination enzyme being isolated with proteus mirabilis CC3-1, the nucleotide with serial number 1. One enzymic protein is generated by gene. One recombinant vector includes gene. Tranformant is transformed by recombinant vector. Transformant is from E.It is select in the special bacterium of coli, streptomyces, the special yeast of saccharomyces, the special yeast of pichia and the special yeast of Rhodotorula. Functional organic acid is by executing the bioconversion generation with the enzyme for the amino acid for being a substrate. Substrate is select from a phenylalanine, isleucine, leucine, tyrosine, valine and lysine.
机译:一种奇异变形杆菌所衍生的氨基酸脱氨酶基因通过与氨基酸底物的生物转化而被提供给大规模生产的各种功能性有机酸。一个基因具有用变形链球菌CC3-1(序列号为1的核苷酸)分离的氨基酸脱氨酶的活性。一种基因产生一种酶蛋白。一种重组载体包括基因。转化子由重组载体转化。转化体来自大肠杆菌。它是从大肠杆菌,链霉菌,酿酒酵母,毕赤酵母和酵母属酵母中选择的。功能性有机酸是通过用氨基酸作为底物的酶进行生物转化来产生的。底物选自苯丙氨酸,异亮氨酸,亮氨酸,酪氨酸,缬氨酸和赖氨酸。

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