Protein chip and detection method of the same comprising protein nanoparticles linked with epitope recognizing disease marker for diagnosis

摘要

The present invention specifically, formed by the self-assembly feature of the ferritin heavy chain relates to a disease diagnostic protein chips, and the disease marker detection method using the same, including the protein nanoparticles is ferritin heavy chain (Ferritin Heavy Chain) and the disease marker specific for an epitope bound that is using a protein nano quantum dot light emission of the second antibody is associated when the disease diagnosis and the disease marker protein chip comprising a detection particle (photoluminescence) relates to a method for detecting disease markers. Protein of the present invention, nanoparticles are made spherical by a self-assembly provide a stable immobilized as an active-type probe effects, with a constant direction above a substrate of the protein chip, and further refers to the effect of improving the degree of integration per unit area of ​​the probe, as well as the applying the ultra-sensitive method for detecting the application of the quantum dot and the PCR of the invention may be useful for developing highly sensitive protein nano-chip development and diagnostic kits. ; Protein nanoparticles, protein chip, ultra-sensitive detection method, diagnosis of disease