Two-Photon Fluorescent Probes for the Acidic Vesicles in Live Cell and Tissue and Method of Imaging the Acidic Vesicles in Live Cell Using the Same

摘要

A two photon dye for imaging the in vivo acidic endoplasmic reticulum is provided to allow the acidic endoplasmic reticulum to be imaged and to allow the acidic endoplasmic reticulum to be monitored in real time. A two photon dye for imaging the in vivo acidic endoplasmic reticulum is represented by the formula 1, wherein R1 is hydrogen or a methoxy group. The two photon dye is excited by the light of a wavelength of 780nm. The two photon dye has a Phidelta of 86 ~ 88GM when excited.