MODIFIED influenza virus, designed to monitor and improve the effectiveness spine VACCINE

摘要

1. hemagglutinin molecule (HA) of influenza virus, comprising an amino acid substitution in the receptor binding site which imparts molecule more antigenic ON with respect to antibodies specific for the HA molecule, which lacks the amino acid substitution in its receptor binding site. ! 2. The influenza virus HA molecule of claim 1 comprising the amino acid asparagine at the position corresponding to position 223 in HA subtype H5 wherein the inclusion of asparagine results in an increased reactivity with antisera derived from an animal that is infected with influenza virus HA molecule comprising wildtype. ! 3. The influenza virus HA molecule of claim 1 comprising the amino acid asparagine at the position corresponding to position 223 in HA H5, wherein the HA molecule does not originate from human H5 isolate A / HK / 213/03, and wherein the inclusion of asparagine at position 223 results in increased reactivity with antisera derived from an animal that is infected with the influenza virus. ! 4. The molecule of influenza virus according to claim 1, wherein the amino acid substitution results in a change in glycosylation site. ! 5. The influenza virus HA molecule of claim 1, wherein the influenza virus is a human influenza A virus. ! 6. The molecule on the influenza virus of claim 5 wherein the human influenza A virus is a member of the H5 subtype. ! 7. The influenza virus HA molecule of claim 5, wherein the human influenza A virus is A / Vietnam / 1203/04 (H5N1). ! 8. The molecule of influenza virus of claim 1 wherein the human influenza virus is an influenza B virus! 9. The molecule of influenza virus according to claim 1, which is derived from avian influenza virus. ! 10. The recombinant influenza virus containing a molecule of influenza virus according to claim 1. !