METHODS OF OBTAINING, SEPARATION AND PURIFYING EPOTHILONE B AND X-RAY CRYSTAL STRUCTURES OF EPOTHILONE B

摘要

FIELD: chemistry.;SUBSTANCE: improved methods of obtaining, separating and purifying epothilone B involve fermentation of microbial strain Sorangium cellulosum and, in particular strain ATCC No. PTA 3880 or ATCC No. PTA 3881, collection of resin with adsorbed epothilone B, solvent extraction for separating epothilone B and crystallisation of the desired product. The method involves obtaining epothilone B derivatives. The invention also relates to strains which produce epothilone B and to a method of culturing these strains to produce epothilone B. Methods are disclosed for purifying epothilone B using high-resolution liquid chromatography with reversed or normal phase.;EFFECT: increased ratio of epothilone B to epothilone A due to introduction of admixtures for fermentation, chosen from propionate, propionic acid or some other propionate precursor, and said purification methods allow for production of virtually pure epothilone B.;45 cl, 19 dwg, 8 tbl, 11 ex