An optical measuring method for determining of the ph - value of a medium under application of ageladine a as a fluorescent ph - value - indicator

摘要

Known fluorescent ph - value - indicators are relatively susceptible to trouble and have only a limited detection range. In the case of the measuring method according to the invention, the marine natural substance ageladine a as a fluorescent ph - - indicator value used. ageladine a is easy to handle, completely synthesized and shows a large ph - value - spectrum with a particular sensitivity in the physiologically particularly relevant region of ph6 to ph8. The under uv - excitation, clear and strong fluorescence occurring in the green range of ageladine a, in spite of the uv - irradiation over hours stable. Living cells, the tissue and the entire, intact organisms, it is possible without damaging influence in a solution having ageladine a rapidly and simply be dyed and incubated while penetrating. The dyeings are held at least over several days. This makes possible, in addition in - vitro - measurements also ph - value - in vivo measurements in a wide range. Acidic constituents of organisms can be immediately after the incubation with ageladine a on a view in the fluorescence - microscope, since the fluorescence of ageladine a with a decreasing ph - value increases strongly.