首页> 外国专利> method for in vitro determination of cellular uptake of exogenous and endogenous substances using nmr and magic angle verschiebungsagentien - nmr technique

method for in vitro determination of cellular uptake of exogenous and endogenous substances using nmr and magic angle verschiebungsagentien - nmr technique

机译：nmr和魔角verschiebungsagentien-nmr技术体外测定外源和内源性物质细胞吸收的方法

页面导航

摘要
著录项
相似文献

摘要

The present invention deals with a method for in vitro quantitative determination of cellular uptake of exogenous substances characterized in that it comprises applying MAS-NMR spectroscopy to an in vitro cellular sample, in combination with a shift agent, and which includes the following steps:1) selection of the shift agent (SA) and nucleus combination to be employed for the measurement of cellular uptake of the exogenous substance (EXO) under investigation;2) determination of the cellular compartment/s (CC/s) in which said exogenous substance (EXO) distributes;3) measurement of said exogenous substance (EXO) compartmental concentration, i.e. of said EXO cellular uptake.

机译：本发明涉及一种体外定量测定外源性物质的细胞摄取的方法，其特征在于，该方法包括在体外细胞样品中应用MAS-NMR光谱。与移位剂结合使用，包括以下步骤： 1）选择用于测量细胞吸收的移动剂（SA）和细胞核组合 2）确定所述外源物质（EXO）分布在其中的细胞室（CC / s）; 2） ListItem> 3）测量所述外源物质（EXO）的隔室浓度，即所述EXO细胞摄取。 展开▼

著录项

公开/公告号DE602005016385D1

专利类型

公开/公告日2009-10-15

原文格式PDF

申请/专利权人 BRACCO IMAGING S.P.A.;
展开▼

申请/专利号DE20056016385T

发明设计人 PALEARI LINO;DEMIRANDA MARIO;CALABI LUISELLA;ALFIERI GOFFREDO;GHELLI STEFANO;BIONDI LUCA;
展开▼

申请日2005-03-22

分类号G01N33/483;G01N24/08;

国家 DE

入库时间 2022-08-21 19:08:07

相似文献

专利

外文文献

中文文献

1. NMR用的标准物质、NMR用的试样管、NMR用的毛细管、及测定试样的NMR的方法 [P] . 中国专利： CN102741700A . 2012-10-17

2. 在体外和体内对工程化的细胞中表达的化学物质诱导的信号传导复合物进行外源性药物激活的方法 [P] . 中国专利： CN110191955A . 2019-08-30

3. method for in vitro determination of cellular uptake of exogenous and endogenous substances using nmr and magic angle verschiebungsagentien - nmr technique [P] . AT441856T . 2009-09-15

机译： nmr和魔角verschiebungsagentien-nmr技术体外测定外源和内源性物质细胞吸收的方法

4. METHOD FOR THE IN VITRO DETERMINATION OF CELLULAR UPTAKE OF EXOGENOUS AND ENDOGENOUS SUBSTANCES USING NMR SHIFT AGENTS AND THE MAGIC ANGLE NMR TECHNIQUE [P] . 欧洲知识产权局专利： EP1735616B1 . 2009-09-02

机译：核磁共振移动试剂和磁角核磁共振技术体外测定外源性和内源性物质的细胞摄取方法

5. Method for the In Vitro Determination of Cellular Uptake of Exogenous and Endogenous Substances Using Nmr Shift Agents and the Magic Angle Nmr Technique [P] . 美国专利： US2008003632A1 . 2008-01-03

机译： Nmr移位剂和魔角Nmr技术体外测定外源和内源性物质的细胞吸收方法

1. Direct Observation of Various Al Sites in SAPO-34 by 27Al Multiquantum (MQ) Magic Angle Spinning (MAS) NMR [J] . 中国化学快报（英文版） . 2003,第001期

2. ~(1)H MAS NMR (Magic-Angle Spinning Nuclear Magnetic Resonance) Techniques for the Quantitative Determination of Hydrogen Types in Solid Catalysts and Supports [J] . GORDON J. KENNEDY, MOBAE AFEWORKI, DAVID C. CALABRO, Applied Spectroscopy: Society for Applied Spectroscopy . 2004,第6期

3. Structure determination of aligned systems by solid-state NMR magic angle spinning methods [J] . Gross BJ, Tanski JM, McDermott AE Journal of magnetic resonance . 2005,第2期

4. Achieving high resolution dipolar NMR information without fast sample spinning: Combining magic angle turning with dipolar based NMR methods [J] . A. Ananthanarayanan, L. van Wullen Solid State Nuclear Magnetic Resonance . 2013,第Null期

5. Methyl Groups in Aquatic Humic Substances: Application of Ultra-Fast Magic Angle Spinning ~1H NMR Spectroscopy [C] . Ulrich Lankes, Fritz H. Frimmel the Meeting of the International Humic Substances Society . 2006

6. Development of an isolated flow variable-temperature magic-angle spinning (MAS) nuclear magnetic resonance (NMR) probe for heterogeneous catalysis studies and high-temperature high-speed 19F MAS NMR techniques applied to fluoropolymers. [D] . Isbester, Paul Kenneth, Jr. 1999

7. Aromatic Spectral Editing Techniques for Magic-Angle-Spinning Solid-State NMR Spectroscopy of Uniformly 13C-Labeled Proteins [O] . Jonathan K. Williams, Klaus Schmidt-Rohr, Mei Hong -1

8. Erratum: Determination of the Si–O–Si bond angle distribution in vitreous silica by magic angle spinning NMR [O] . R. Dupree, R. F. Pettifer 1984

1. STEREOSTRUCTURAL DETERMINATION OF ADDITION PRODUCTS FROM ISOFURANOGERMACRENE AND MALEIMIDE BY NMR TECHNIQUES [J] . Xiu Wen HAN~* Man KONG~+ Wen Yi HE~+ Chang Fu XU~+ Dalian Institute of Chemical Physics Academia Sinica Dalian 116023 +: Beijing Institute of Materia Medica Chinese Academy of Medical Sciences Beijing 100050 Shi Zhen MAO Xiao Long XU Yan Wu YANG Guo Xi WANG Magnetic Resonance and Atomic and Molecular Laboratory Wuhan 430071 . 中国化学快报：英文版 . 1992,第11期

2. APPLICATION OF 2D-NMR TECHNIQUES IN THE STRUCTURE DETERMINATION OF HYPODEMATINE [J] . Ya Min CHEN Ying Hua LU Yan Jun CHEN Cun Sheng MA (Jining Medical CollegeShandong272113) De Quan YU (Institute of Materia MedicaChinese Academy of Medical SciencesBeijing100050) . 中国化学快报：英文版 . 1990,第3期

3. Use of Magic Angle Spinning ~1H NMR Spectroscopy to Monitor Reactions in Solid-Phase Synthesis [J] . Ai Ming YU ,Hua Zheng YANG(The State Key Laboratory of Elemento-Organic Chemistry Nankai University Tianjin 300071)Ping Chuan SUN ,Zhen Pu ZHANG . 中国化学快报：英文版 . 1997,第12期

4. ^(29)Si, ^(27)Al Magic-Angle-Spinning Nuclear Magnetic Resonance (MAS NMR) Studies of Kaolinite and Its Thermal Transformation Products [J] . 何宏平 ,胡澄 ,郭九皋 . 地球化学学报（英文） . 1995,第1期

5. 急性肾损伤患儿内源性促红细胞生成素的变化及外源性促红细胞生成素对肾脏的保护作用 [C] . 李志辉 ,张良 ,张翼 . 中华医学会第十八次全国儿科学术会议 . 2013

6. Ⅰ高分辨魔角核磁共振技术（HR/MAS NMR）在组合化学中的应用;ⅡN-去甲基万古霉素衍生物的固相合成及其抗菌活性研究 [A] . 姚念环 . 2004