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METHOD AND KIT FOR TESTING DRUG SENSIBILITY OF TUBERCLE BACILLUS STRAIN
METHOD AND KIT FOR TESTING DRUG SENSIBILITY OF TUBERCLE BACILLUS STRAIN
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机译:测试结核杆菌芽胞杆菌药敏性的方法和试剂盒
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摘要
PROBLEM TO BE SOLVED: To provide a method for testing the drug sensibility of a tubercle bacillus, constituted so as to be simply and rapidly performed using a small amount of a culture medium and enhanced in safety, and also to provide a kit for testing the drug sensibility of the tubercle bacillus.;SOLUTION: In the method for testing the drug sensibility of the tubercle bacillus, a specimen is cultured in a culture medium containing a tuberculostat and the obtained cultured medium is subjected to immunoassay to detect the tubercle bacillus strain specific secretory protein in the cultured medium as a tubercle bacillus marker. The specimen is cultured in a plurality of culture mediums containing tuberculostats different from each other so as to detect a multi-drug resistant tubercle bacillus and the obtained respective cultured mediums are preferably subjected to immunoassay. As the tuberculostat, isoniazid (INH), rifampicin (RFP), streptomycin (SM), ethambutol (EB), kanamycin (KM) or the like is used. As a control, it is preferable that the specimen is cultured in a culture medium containing no tuberculostat and the obtained cultured medium is preferably subjected to immunoassay. The immunoassay is preferably sandwich type immunoassay such as immunochromatography assay or the like.;COPYRIGHT: (C)2010,JPO&INPIT
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