MEMBRANE PROTEIN EXPRESSION SYSTEM AND ITS APPLICATION IN DRUG SCREENING

摘要

PPROBLEM TO BE SOLVED: To provide a convenient in vitro membrane protein expression system using an inhibitor of drug efflux pump proteins and heterologous cells for testing other drug resistance-relating cell membrane proteins. PSOLUTION: Provided is a protein expression system comprising an in vitro cell-based expression system for over-expression of a heterologous protein having efflux pump action relating to the drug resistance of a host cell membrane for drug screening applications, that is to say, a protein expression system comprising: (i) a host yeast cell; and (ii) a vector comprising the coding sequence of a target heterologous membrane protein, wherein the sequence is under the control of a promoter which, upon transformation of the host cell and chromosomal integration, causes over-expression of the functional target protein in the membrane of the host cell. PCOPYRIGHT: (C)2010,JPO&INPIT