METHOD OF DETERMINING ANTIGEN-BINDING SITE IN ANTI-MEMBRANE PENETRATION TYPE PROTEIN ANTIBODY

摘要

PROBLEM TO BE SOLVED: To provide a method of conveniently and efficiently determining a binding site of an antibody to a membrane penetration type protein.;SOLUTION: The method of determining an antigen-binding site of the antibody to the membrane penetration type protein having two or more extramembranous regions includes: the process for expressing a group of variants having different extramembranous region and one or more added amino acid variants by a budding yeast expression system; the process for considering a group of the variants expressed by the budding yeast expression system as the antigen, making the antibody react to the membrane penetration type protein, and implementing an antigen-antibody reaction; and the process for identifying the variants in which the antigen-antibody reaction is not detected, and determining the extramembranous regions in which the variants are varied as a region including the antigen-binding site of the antibody or the antigen-binding site. The method determines the antigen-binding site of the antibody to the membrane penetration type protein.;COPYRIGHT: (C)2010,JPO&INPIT