Amino acid sequencing methods of the amino terminus of the protein

摘要

PROBLEM TO BE SOLVED: To make determinable an amino acid sequence by chemically/ biochemically cutting protein having a modified amino-terminal, allowing carboxylic acid anhydride or an isocyanate compound to act on the obtained peptide, analyzing the mass of peptide before and after the action of carboxylic acid anhydride or isocyanate compound, and comparing the results to specify peptide.;SOLUTION: A protein having a modified amino-terminal is cut by a chemical/biochemical means, carboxylic acid anhydride represented by (R1CO)2O is made to act on the obtained peptide when the pH is below 5, and an isocyanate compound represented by R2 NCO is acted on the same when pH is below 6. In the formulas, R1 is an alkyl group, R2 is an optionally substituted alkyl group, phenyl group, naphthyl group or pyridyl group. The mass of peptide before and after the action is analyzed, the results are compared to specify peptide derived from an amino-terminal of protein, and the amino acid sequence of a specified peptide is determined. Thereby, N-end amino acid can be modified with high selectivity even in the co-existence of ε-amino group.;COPYRIGHT: (C)2000,JPO