Extracellular expression of a cellulose binding domain using Bacillus subtilis

摘要

(57) Abstract Character it is converted by the vector which possesses the DNA array which cellulose join domain (CBD) the code is done, regards the bacillus germ host who at the same time can reveal that array. That the polypeptide which is revealed consists of the non catalytic converter domain 1 or more essentially, the molecular weight of this cellulose join domain is the range 4 - 35kD, from the microbe or the plant, desirably it is obtained from the bacterium or the true germ, this bacillus germ host, for example is one germ genus of Bacillus subtilis, bacillus raikenihorumisu, Bacillus megaterium, bacillus sutearosamohuirosu and bacillus amirorikuehueishiensu. And, the code is done the DNA array which is interposed this cellulose join domain and regards the manner which produces the cellulose join domain polypeptide in the manifestation vector, and the bacillus germ host cell for the bacillus germ which is containment.