Human neuronal acid-sensitive cation channel, its cloning, and apply

摘要

(57) [summary] do not inactivate, or proton-gated cation channels that inactivate slowly, are thought to play an important role in pain perception to occur in association with the organization acidosis. The present inventors have identified a biphasic desensitization kinetics with a persistence component Na ⁺ selectivity components rapidly inactivated, human proton-gated cation channel subunit. Sequence identity with this protein, proton-dependent cation channel rASIC3 derived from rat sensory neurons and (rDRASIC) is 84%. From sequence homology and biphasic desensitization kinetics this, that it is a human ortholog of rASIC3 (rDRASIC) is suggested (hASIC3) this clone. rASIC3 (rDRASIC) requires acidic pH (pH4.5) for very persistent current activation but, on the other hand, hASIC3 current which does not inactivate the activation pH is reduced to pH6 below begins to be. hASIC3 is an acid sensor, might play an important role in the detection of pH changes persistent in humans. The inventors have found that the position of hASIC3 gene, located on human chromosome 7q35 in 6.4cRad telomeric from the microsatellite AFMA082XC9.