Probe and its use for mutation detection of JAK2 gene

摘要

The detection object arrangement which includes the aforementioned mutation the probe for detection of detection possible JAK2 gene mutation is offered even with when coexist with the detection object arrangement where only one base differs, includes mutation and mutation the non detection object arrangement which is not included. The 84th cytosine base in exon12 of the JAK2 gene which consists of the base arrangement of arrangement number 1 (C) as the first base 17 - as the territory to the 22nd base at least being one which is the same arrangement origonukureochido 5 ' facing toward direction, the aforementioned cytosine base (C) 3 ' you use origonukureochido which is made end as the probe. These probes, for example, being the sample where the JAK2 gene where mutation occurs by using for Tm analysis, and the JAK2 gene where mutation does not occur are included, it is possible to detect the mutation of former. As for the aforementioned probe, to sign it is desirable with the fluorescence dye to be converted.