Composition for repairing skin or gingival soft tissue defects, and method for culturing autologous fibroblasts

摘要

PROBLEM TO BE SOLVED: To provide a method for producing an aggregate of self-fibroblasts for producing self-fibroblasts aggregated in a filament form, and to provide an aggregate produced by such a method. . In addition, the present invention comprises a composition for repairing a skin or gingival soft tissue defect that can maintain an effective therapeutic effect by staying at the treatment site for a long period of time, or such a composition. An object is to provide an injection. The object is to cultivate autologous fibroblasts in a culture medium to which vitamin C is added, and to form self-fibroblasts in a filamentous form by an extracellular matrix containing collagen secreted by the autologous fibroblasts. Solution by a method for producing an aggregate of autologous fibroblasts, including a step of culturing by any one of rotational culture, shaking culture, or gradient culture, and the aggregate to be produced. Is done. [Selection] Figure 7